Search results for "Trypsin Digestion"
showing 2 items of 2 documents
Recognition and alignment of variables from UV–vis chromatograms and application to industrial enzyme digests classification
2017
Abstract In the last years, industrial applications of chemometrics have largely increased due to their capacity to extract important information from complex records as chromatograms or spectra data. The use of chemometric methods also can avoid the use of detectors of elevated cost. In this work, a procedure to recognize the relevant chemical information contained in complex UV–vis chromatograms, after a trypsin digestion, to identify the three enzyme main classes (proteases, amylases and cellulases) commonly employed in the cleaning industry, has been developed. In order to recognize the chromatogram peaks, six indices of peak identity or identifiers were defined. A program written in MA…
Comparison of monolithic and microparticulate columns for reversed-phase liquid chromatography of tryptic digests of industrial enzymes in cleaning p…
2011
Abstract Enzymes of several classes used in the formulations of cleaning products were characterized by trypsin digestion followed by HPLC with UV detection. A polymeric monolithic column (ProSwift) was used to optimize the separation of both the intact enzymes and their tryptic digests. This column was adequate for the quality control of raw industrial enzyme concentrates. Then, monolithic and microparticulate columns were compared for peptide analysis. Under optimized conditions, the analysis of tryptic digests of enzymes of different classes commonly used in the formulation of cleaning products was carried out. Number of peaks, peak capacity and global resolution were obtained in order t…